Human IL1B gene induction requires transcription factor‐mediated DNA looping
نویسندگان
چکیده
منابع مشابه
DNA binding by GATA transcription factor suggests mechanisms of DNA looping and long-range gene regulation.
GATA transcription factors regulate transcription during development and differentiation by recognizing distinct GATA sites with a tandem of two conserved zinc fingers, and by mediating long-range DNA looping. However, the molecular basis of these processes is not well understood. Here, we determined three crystal structures of the full DNA-binding domain (DBD) of human GATA3 protein, which con...
متن کاملProtein-mediated looping of DNA under tension requires supercoiling
Protein-mediated DNA looping is ubiquitous in chromatin organization and gene regulation, but to what extent supercoiling or nucleoid associated proteins promote looping is poorly understood. Using the lac repressor (LacI), a paradigmatic loop-mediating protein, we measured LacI-induced looping as a function of either supercoiling or the concentration of the HU protein, an abundant nucleoid pro...
متن کاملDNA looping and physical constraints on transcription regulation.
DNA looping participates in transcriptional regulation, for instance, by allowing distal binding sites to act synergistically. Here, we study this process and compare different regulatory mechanisms based on repression with and without looping. Within a simple mathematical model for the lac operon, we show that regulation based on DNA looping, in addition to increasing the repression level, can...
متن کاملSequence Dependence of Transcription Factor-Mediated DNA Looping: Supplementary Material
S1 Modeling the Looping Probability. 2 S1.1 Tuning the simple titration curve. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 S1.2 The case of multiple looped states. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 S1.3 Effect of an “inactive fraction” of repressor. . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 S1.4 Effect of the presence of dimers...
متن کاملDNA looping.
DNA looping is widely used in nature. It is well documented in the regulation of prokaryotic and eukaryotic gene expression, DNA replication, and site-specific DNA recombination. Undoubtedly looping also functions in other protein-DNA transactions such as repair and chromosome segregation. While the underlying physical chemistry of DNA looping is common to all systems, the precise biochemical d...
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ژورنال
عنوان ژورنال: The FASEB Journal
سال: 2009
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fasebj.23.1_supplement.487.6